rabbit polyclonal gdf9 antibody  (Bioss)

Journal: Antioxidants

Article Title: Tannin Reduces the Incidence of Polyspermic Penetration in Porcine Oocytes

doi: 10.3390/antiox11102027

Figure Lengend Snippet: Detection of GDF9 and BMP15 proteins by immunofluorescence staining and detection of p-CKD1 and CKD1 proteins by Western blot in oocytes. ( A , B ) GDF9 protein expression. ( C , D ) BMP15 protein expression. ( E ) p-CDK1 and CDK1 protein expression. Within the same indicator, bars with different letters indicate a significant difference ( p < 0.05). Results are shown as the average ± SEM of at least three repeats of independent experiments. A total of 30 oocytes were analyzed per group in each replicate.

Article Snippet: Porcine oocytes were incubated with rabbit polyclonal antibodies against GDF9 (ab93892; Abcam, Cambridge, UK) and BMP15 (PA5-34401; Invitrogen) at 37 °C for 2 h. The samples were washed three times in 2% BSA and then incubated with a goat anti-rabbit fluorescein isothiocyanate-conjugated secondary antibody (1:200; Jackson Immuno Research Laboratories Inc., West Grove, PA, USA) for 2 h. Oocytes were mounted on glass slides and the images were captured using an epifluorescence microscope (TE2000-S; Nikon).

Techniques: Immunofluorescence, Staining, Western Blot, Expressing

Journal: Cell Death & Disease

Article Title: CPEB3 deficiency in mice affect ovarian follicle development and causes premature ovarian insufficiency

doi: 10.1038/s41419-021-04374-4

Figure Lengend Snippet: A Western blot analysis of GAPDH, GDF9, PCNA, and Cleaved-Caspase3 protein expression in 3-month-old WT and Cpeb3 -mutant ovaries. GAPDH was used as the internal control. B Western blot analysis of GDF9 and GAPDH in WT and Cpeb3 -mutant oocytes. GAPDH was used as the internal control. C Ovarian sections stained for GDF9 at 3-month-old WT and Cpeb3 -mutant ovaries. D Gdf9 mRNA levels were performed by qRT-PCR in WT and Cpeb3- mutant oocytes. Gapdh was used as the internal control. Scale bar = 50 μm and 25 μm in C . *** P < 0.001.

Article Snippet: Rabbit polyclonal anti-GDF9 antibody was purchased from Abcam (ab93892).

Techniques: Western Blot, Expressing, Mutagenesis, Control, Staining, Quantitative RT-PCR